DEVELOPMENT OF ANTIBODY TO RALSTONIA SOLANACEARUM AND ITS APPLICATION FOR DETECTION OF BACTERIAL WILT

YADI SURYADI

Abstract


The serological assay for  the detection of  bacterial wilt caused by Ralstonia  solanacearum  (RS)
was able to provide  information regarding the presence of  the pathogen  in plant materials. The
research is was aimed to develop polyclonal antibody (PAb) for RS detection. Bacterial whole
cells of  RS isolates mixed with glutaraldehyde were used to immunize New Zealand female
white  rabbit. The  titre of  antibody  in culture supernatant was 1: 1024. The PAb developed  from
a ground nut RS  isolates reacted with  infected plant samples from various  locations. It was able
to detect RS antigen of  crude extract and pure cultures from tomato and potato plant samples
4-5
using dot blot ELISA; however, the minimum detectable concentration of  RS antigen was 10
cells/ml. The PAb obtained in this study is sensitive enough to detect RS isolates in routine
serological assay.
Key words:  Bacterial wilt, Ralstonia  solanacearum, antibody, dot blot ELISA.

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DOI: http://dx.doi.org/10.11598/btb.2009.16.2.58

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