DIVERSITY OF ACTINOMYCETES FROM EKA KARYA BOTANICAL GARDEN, BALI

Shanti Ratnakomala, Puspita Lisdiyanti, Nita Rosalinda Prayitno, Evi Triana, Yulin Lestari, Ratih Dwi Hastuti, Yantyati Widyastuti, Misa Otoguro, Katsuhiko Ando, Endang Sukara

Abstract


A total of 229 strains of actinomycetes were isolated and identified by full sequence of 16S rRNA gene analysis. Samples consisted of 18 soil and 20 leaf-litter were collected from Eka Karya Botanical Garden, Bali Island, Indonesia. Two isolation methods, i.e. SDS-Yeast Extract (SY) and Rehydration-Centrifugation (RC) were used in this study. Based on 16S rRNA gene analysis, isolated actinomycetes may be grouped into 28 genera. Based on molecular analysis of 16S rRNA gene similarities showed that isolated actinomycetes of Eka Karya Botanical Garden origin is diverse. Analysis on 144 isolates from soil samples, resulted in 24 genera and more than 87 species. Streptomyces is the most dominant genus where 65 isolates or 45% from isolated actinomycetes belong to this genus. It was followed by Actinoplanes (25 isolates =17%). From leaf-littersamples, the total number of 85 isolates may be grouped into 9 genera and more than 41 species. The most dominated genus is Actinoplanes (42 isolates =49%) followed by Catenuloplanes (16 isolates=19%).

Keywords: 16S rRNA gene analysis, actinomycetes, biodiversity, Eka Karya Botanical Garden


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DOI: http://dx.doi.org/10.11598/btb.2016.23.1.504

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