PRODUCTION AND UTILIZATION OF CELLULASE FROM TRICHODERMA VIRIDE

SURYAHADI SURYAHADI, R. HIDAYAT, R. HIDAYAT, S. WULANDARI, S. WULANDARI, K.G. WIRYAWAN, K.G. WIRYAWAN

Abstract



  An appropriate preservation technology for forage such as silage needs to be developed in order to
overcome the shortage of tropical forage during the dry season. A good quality of silage is obtained by
decreasing the fibre contents (Neutral Detergent Fibre, Acid Detergent Fibre and lignin). The research
was conducted in two stages: 1). Production and activity test of crude enzymes from Trichoderma viride
and 2). Comparative test using crude enzymes from  Trichoderma viride strain QM 9414 (CETV) and
commercial cellulase (Cellulase “Onozuka
R-10”, heidelberg) on rice straw silage. The treatments were
arranged as follows : P0=Untreated rice straw silage, P1=Treated with commercial cellulase at 10 IU kg-1
,
P2= Treated with CETV at 3.8 IU kg-1
, P3=Treated with CETV at 7.6 IU kg-1
  and P4= Treated with
CETV at 11.4 IU kg-1
 of fresh rice straw. All treatments were enriched with 5% molasses. The result of
the research indicated that: 1). The activity of crude enzymes from Trichoderma viride strain QM 9414
(CETV) was 1.52 IUml
-1
. 2). The addition of both enzymes did not decrease dry matter of rice straw
silage; however, organic matter significantly decreased (p<0.05) by addition of commercial cellulase. 3).
The addition of both enzymes decreased fibre contents (NDF), furthermore increased (p<0.05)  in sacco
digestibility of dry matter and organic matter of rice straw silage.
 
Keywords  :  Cellulase/Trichoderma viride/Rice straw/Silage/Fibre

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DOI: http://dx.doi.org/10.11598/btb.2005.0.25.208

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