PRESERVATION OF GARUT RAMS SPERMATOZOON AS A SOURCE OF MALE GERM PLASM

ARIEF BOEDIONO, HERDIS , MUHAMMAD RIZAL'

Abstract


This study was conducted to examine the quality of ejaculated sperm by Garut rams to be used for artificial
insemination (AI) and viability of sperm that were collected from preserved cauda epididymis  (4°C up to 12 days) for
assisted reproductive technology. The semen was collected by artificial vagina, with the sperm motility, live sperm,
acrosomal intact, and intact plasma membrane observed. Sperm motility was 75%, while for the live sperm, intact plasma
membrane and sperm abnormality were 91.5%, 90.0%, and 1.8%, respectively. In the other study, sperm was collected
from cauda epididymis by aspiration method and diluted in different media: 1) Brackett Oliphant (BO) media and 2)
modified Phosphate Buffer Saline (mPBS). Evaluation of  sperm motility and intact plasma membrane were
conducted after washing, counting and dilution of the sperm. The results of this study showed that the sperm motility and
intact plasma membrane could be maintained better in BO rather than PBS medium although they were not statistically
different (P>0.05). At day 12 of preservation, the motility and intact plasma membrane of sperm collected from cauda
epididymis were 0.7% and 1.33% for motility and plasma membrane intact, respectively. These findings showed that the
Garut rams semen was qualified for AI and frozen processing; in vitro embryo production by introducing the assisted
reproductive technology such as intracytoplasmic sperm injection (ICSI) could be applied by using the sperm collected from
preserved cauda epididymis until 12 days of preservation at 4°C.
Keywords :   Reproduction/spcrmatogenesis/inscmination/Garut rams/small ruminant

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DOI: http://dx.doi.org/10.11598/btb.2004.0.23.200

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