INFLUENCE OF MEVALONIC ACID AND LINALOOL ON LIMONENE ACCUMULATION IN CALLUS TISSUES OF CITRUS GRANDIS OSBECK

ZARINA ZAKARIA, NIK NORULAINI NIK AB RAHMAN, MOHD OMAR ADD KADIRS

Abstract



Effect on callus growth was studied for Citrus grandis  cultured with feeding of exogenous mevalonic acid
(MVA) at concentrations of 0.04, 0.08, 0.38, 0.77, 1.15 and 1.54 mM. Similar effect with linalool ranging from 10 to 200
nl was studied under various incubation periods. The growth was proportional to the concentrations of precursors
used meaning that higher precursors concentrations influenced more growth on C. grandis callus culture. Mevalonic acid
and linalool showed quite similar precursor feeding effects on limonene accumulation of C. grandis callus cultures. It was
revealed that limonene production was triggered with the introduction of MVA and linalool even at low concentration.
Limonene accumulation was detected as early as week four and continued to increase at about 0.0030 and 0.0032 mg/g with
MVA and linalool, respectively, after the seventh week incubation. In comparison to the unfed cultures, no limonene was
detected from the callus up to eight weeks in incubation.
Keywords :   C/rrusgrarafo/Limonene/Linalool/Mevalonic acid
Abbreviations: 2,4-D: 2,4 — Dichlorophenoxyacetic acid, ABA: Abscisic acid, MVA : Mevalonic acid

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DOI: http://dx.doi.org/10.11598/btb.2003.0.20.183

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