CHARACTERIZATION OF XYLANASE FROM A XYLANOLYTIC- THERMOPHILIC BACTERIUM ISOLATED FROM GUNUNG PANCAR HOT SPRING, WEST JAVA
DOI:
https://doi.org/10.11598/btb.2001.0.17.167Abstract
A xylanolytic-thermophilic bacterium (IT-08) was isolated from Gunung Pancar Hot Spring after twodays of enrichment in Modified Thermus Medium (MTM) supplemented with 0.5% oat spelt xylan. 16S-
rRNA sequence analysis indicated that IT-08 resembles Bacillus thermoleovorans, a species of thermophilic
bacteria. When grown on xylan containing media, IT-08 produces a thermoactive xylanase. Xylanase from IT-
08 was active at temperatures between 40 and 100°C, at pH values between 4.0 and 9.0 with optimum values
obtained at 80°C and pH 6.0, respectively. SDS-PAGE and zymogram analysis demonstrated that a crude
xylanase complex of IT-08 comprised two active bands with molecular masses of 78 and 60 kDa.
Keywords: xylanase / xylanolytic-thermophilic bacteria
Downloads
Download data is not yet available.
Downloads
How to Cite
SUWANTO, A., SURYANTO, D., & MERYANDINI, A. (2011). CHARACTERIZATION OF XYLANASE FROM A XYLANOLYTIC- THERMOPHILIC BACTERIUM ISOLATED FROM GUNUNG PANCAR HOT SPRING, WEST JAVA. BIOTROPIA, (17). https://doi.org/10.11598/btb.2001.0.17.167
Issue
Section
Research Paper
License
Authors who publish with this journal agree with the following terms:
- Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivates 4.0 International License that allows others to share, copy and redistribute the work in any medium or format, but only where the use is for non-commercial purposes and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
