CHARACTERIZATION OF STREPTOMYCES sp. 45I-3 XYLANASE

Anja Meryandini, Deden Saprudin, Prima Agung Prihandono, Alina Akhdiya, Trio Hendarwin

Abstract


Streptomyces sp 45I-3 producing xylanase was isolated from Kalimantan soil sample. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase has an optimum condition at pH 5 and 50 oC.  Crude xylanase have half-life time of 2 hours 9 minute at 50 oC, whereas purified xylanase has half-life time of 12 hours. The molecular mass of purified xylanase was determined to be 43.2 and 39.2 kDa.


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DOI: http://dx.doi.org/10.11598/btb.2007.14.2.16

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