PHYTASE PRODUCTION BY Enterobacter cloacae

Authors

  • Suliasih Suliasih Bidang Mikrobiologi-Puslit Biologi-LIPI
  • Sri Widawati

DOI:

https://doi.org/10.11598/btb.0.0.0.1233

Keywords:

Phytase producing bacteria, Enterobacter cloacae, phytase

Abstract

 

This study aims to isolate the phytase producing bacteria (PPB), a plant growth promoting rhizobacteria (PGPR), from 1Cigna sinensis rhizosphere and to optimize its physicochemical conditioning. Phytase is an enzyme that can hydrolyze the phosphoester bond in organic phosphorus (phytic acid) to form ester phosphate and inorganic phosphate, the available forms of phosphorus. To test its ability to hydrolyze organic phosphates (calcium phytate), the phytase was screened in solid and liquid phytase screening medium (PSM). After isolation, a total of 13 bacteria were positive for this enzyme’s production as indicated by the clear zones of hydrolysis observed around the colony. Enterobacter cloacae strain B1 had the largest hydrolysis efficient (3.43) on solid medium. The phytase-production of the Enterobacter cloacae strain grown in liquid PSM, showed 0.92 U/mL after 48 hours of incubation. This strain produced optimum levels of phytase in the presence of lactose and monoammonium phosphate (NH4H2PO4), as carbon and nitrogen sources, respectively, at 30 °C and pH 5.0. The PPB obtained in this study are recommended for further research as to their use as plant biological fertilizers.

 

 

Downloads

Download data is not yet available.

Author Biography

Suliasih Suliasih, Bidang Mikrobiologi-Puslit Biologi-LIPI

Research center

Downloads

Published

2020-11-23

How to Cite

Suliasih, S., & Widawati, S. (2020). PHYTASE PRODUCTION BY Enterobacter cloacae. BIOTROPIA, 27(3), 282–291. https://doi.org/10.11598/btb.0.0.0.1233

Issue

Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020

Section

Research Paper

License

Authors who publish with this journal agree with the following terms:

  1. Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivates 4.0 International License that allows others to share, copy and redistribute the work in any medium or format, but only where the use is for non-commercial purposes and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).