GENETICS IDENTIFICATION OF SEA HOLLY (Acanthus ilicifolius) THROUGH DNA BARCODING FROM COASTAL CILACAP, CENTRAL JAVA, INDONESIA

Taufan Harisam, Florencius Eko Dwi Haryono, Bintang Marhaeni, Amron Amron, Norman Arie Prayogo, Purnama Sukardi

Abstract


Information on molecular taxonomy of Sea Holly (A. ilicifolius) in coastal Cilacap, Central Java, Indonesia, was very limited. The present study aims to identified Sea Holly (A. ilicifolius) in coastal Cilacap. This study produced a reference library of molecular for Sea Holly (A. ilicifolius). The recently recorded species were utilized for the barcoding investigation. Genetic identification were evaluated by rbcL and matK gene. The young leaf samples of A. ilicifolius was extracted for DNA isolation and amplified with rbcL and matK primer. The length of rbcL gene was 608 bp, and the matK gene was 970 bp.The evolutionary history was build using the Neighbor-Joining Method. The barcodes sequences rbcL and matK analyzed with BLAST and MULTALIN. The sequences were also submitted to NCBI.  From this study, we found that genera of Acanthus (Acanthaceae) and other genera were clustered in the same clade with high bootstrap value. The result indicated that locus of rbcL and matK gene cannot be used for species differentiation in Acanthus, however, these genes can be used for distinguishing the genus level within Acanthaceae.


Keywords


A. ilicifolius, DNA barcode, matK, rbcL, sea holly

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DOI: http://dx.doi.org/10.11598/btb.2020.27.1.1105

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